Bar code assay for DNA



"Bio-Bar-Code-Based DNA Detection with PCR-like Sensitivity," J. Amer. Chem. Soc., 126, 5932-5933 (2004).

After NP probes mixed with bar-code DNA solution were added to a bar-code capture DNA-modified chip, the spots on a chip were labeled with NP probes and bar-code DNA strands. The spotted chip was then exposed to silver enhancement solution for further signal enhancement. The developed spots were then read by measuring the scattered light from the developed spots and provides a permanent record for the assay. The test shows a linear relationship between target concentration and spot intensity over a 4 orders of magnitude concentration range.